Analysis Note

ControlHuman foreskin fibroblasts (SCC058)

Application

Research Sub CategoryPluripotent & Early DifferentiationDevelopmental NeuroscienceMesenchymal Stem CellsNeurodegenerative Diseases

Evaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in H9 human embryonic stem cells. Evaluated by Immunocytochemistry in mouse embryonic fibroblasts. Immunocytochemistry Analysis: A 1:50 dilution of this antibody did not detect nuclei in mouse embryonic fibroblasts.

The anti-Nuclei, clone 235-1, Cy3 conjugate will recognize human cell nuclei only & is usefull in xenograph transplantation studies to study stem cell migration.

Research CategoryStem Cell ResearchStem Cell Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat.

Immunogen

Human nuclei

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Quality

Evaluated by Immunocytochemistry in human foreskin fibroblasts (SCC058). Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected nuclei in human foreskin fibroblasts (SCC058).

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.